Description

Lipid-Depleted Fetal Bovine Serum (LD-FBS)

Background

The analysis of lipid metabolism in cultured cells is challenging. That is because the cell culture carries out in presence of animal serum, which contains a wide range of undefined lipid species. Generally, the best used for Lipid-Depleted Fetal Bovine Serum (LD-FBS) in research applications is:

1. When the normal concentration of lipids may interfere with the results.
2. For studies directly involve lipid metabolism. That is because the composition of serum can have a profound impact on important cellular checkpoints.
3. It is used for the generation of media with defined lipid species.

Special Features

• PurMa^TM Premium LD-FBS is of USA origin
• Additionally, has gone through  an endotoxin depletion procedure.
• Has gone through gamma irradiation as well as heat inactivation process.
• PurMa Biologics performs the most sophisticated lipid depletion.
• Th process will continue with dialysis using the 3500 Dalton dialysis tube.
• The dialysis carries out against 5X 10 Liters of PBD with physiological pH per each 500 ml of serum.
• The procedure depletes the lipids, phospholipids, and a long chain of fatty acids.
• Lastly, we vigorously analyze various aspects, and each LOT possesses a certificate of analysis.
• The process eliminate mycoplasma, IgG, steroids and hormones, and other pathogens.
• Acceptable level of Endotoxin hemoglobin (20 mg/DL) and endotoxin (10 mg/DL).

Analysis

An example of results following PurMa™ FBS Lipid depletion procedure : The Lipid Analysis of Lot: 30143050032423 following Lipid Depletion

For non-proprietary information in PurMa biologics’ general procedure for FBS Lipid depletion, click here:  PurMa™ FBS Lipid depletion Procedure

Routine pathological analysis on PurMa bovine and horse serums: Routine Patalogicsl Analysis on PurMa Bovine and Horse Serums

References

1. Effect of the removal of lipids on specific precipitation. Krueger et al. J Exp Med. 1950 Oct 31; 92(5): 383–391. doi: 10.1084/jem.92.5.383. PMCID: PMC2135985.
2. Preparation of Lipid-Stripped Serum for the Study of Lipid Metabolism in Cell Culture. Aaron et al. 2018 Jun 5; 8(11): e2876. Published online 2018 Jun 5.doi:10.21769/Bio Pro toc.2876, PMCID: PMC8275250.