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Lipid-Depleted Fetal Bovine Serum (LD-FBS)

Lipid-Depleted Fetal Bovine Serum (LD-FBS)

(18 customer reviews)

Generally, the best used for Lipid-Depleted Fetal Bovine Serum (LD-FBS) in research applications is when the normal concentration of lipids may interfere with the results. moreover, For studies directly involve lipid metabolism. That is because the composition of serum can have a profound impact on important cellular checkpoints. It is also used for the generation of media with defined lipid species.

SKU: N/A Categories: Bovine Serums, Fetal Bovine Serum Tags: Fetal Bovine Serum, Lipid-Depleted

Description

Background

The analysis of lipid metabolism in cultured cells is challenging. That is because the cell culture carries out in presence of animal serum, which contains a wide range of undefined lipid species. Generally, the best used for Lipid-Depleted Fetal Bovine Serum (LD-FBS) in research applications is:

  1. When the normal concentration of lipids may interfere with the results.
  2. For studies directly involve lipid metabolism. That is because the composition of serum can have a profound impact on important cellular checkpoints.
  3. It is used for the generation of media with defined lipid species.

Special Features

  • PurMaTM Premium LD-FBS is of USA origin
  • Additionally, has gone through  an endotoxin depletion procedure.
  • Has gone through gamma irradiation as well as heat inactivation process.
  • PurMa Biologics performs the most sophisticated lipid depletion.
  • Th process will continue with dialysis using the 3500 Dalton dialysis tube.
  • The dialysis carries out against 5X 10 Liters of PBD with physiological pH per each 500 ml of serum.
  • The procedure depletes the lipids, phospholipids, and a long chain of fatty acids.
  • Lastly, we vigorously analyze various aspects, and each LOT possesses a certificate of analysis.
  • The process eliminate mycoplasma, IgG, steroids and hormones, and other pathogens.
  • Acceptable level of Endotoxin hemoglobin (20 mg/DL) and endotoxin (10 mg/DL).

Analysis

An example of results following PurMa™ FBS Lipid depletion procedure : The Lipid Analysis of Lot: 30143050032423 following Lipid Depletion

For non-proprietary information in PurMa biologics’ general procedure for FBS Lipid depletion, click here:  PurMa™ FBS Lipid depletion Procedure

Routine pathological analysis on PurMa bovine and horse serums: Routine Patalogicsl Analysis on PurMa Bovine and Horse Serums

References

  1. Effect of the removal of lipids on specific precipitation. Krueger et al. J Exp Med. 1950 Oct 31; 92(5): 383–391. doi: 10.1084/jem.92.5.383. PMCID: PMC2135985.
  2. Preparation of Lipid-Stripped Serum for the Study of Lipid Metabolism in Cell Culture. Aaron et al. 2018 Jun 5; 8(11): e2876. Published online 2018 Jun 5.doi:10.21769/Bio Pro toc.2876, PMCID: PMC8275250.

 

Methodology Unit measured Accepted level Result
Sterility (Current USP and EP 2.6.1 for Bacteria & Fungi) NA Not Detected Not Detected
pH N/A measured 6.58
Osmolality (USP 785)     mOsm/KgH2 mOsm/KgH20 280-340 288
Hemoglobin (Fleming & Woolf) mg/dL <25 12.9
Endotoxin (USP 85) EU/mL <20 <0.100
Mycoplasma (Barile & Kern; Large Volume, Direct Culture) NA Not Detected Not Detected
IgG Elliza Measured 0.19 ug/ml
Albumin Eliza Measured ≤ 4.6 g/dL
Virus Testing (9 CFR 113.53c)
Bovine Adenovirus NA Not Detected Not Detected
Bovine Parvovirus NA Not Detected Not Detected
Bovine Respiratory Syncytial Virus NA Not Detected Not Detected
Bovine Viral Diarrhea Virus NA Not Detected Not Detected
Rabies NA Not Detected Not Detected
Reovirus NA Not Detected Not Detected
Cytopathogenic Agents (IBR) NA Not Detected Not Detected
Hemadsorbing Agents (PI3) NA Not Detected Not Detected
Bluetongue NA Not Detected Not Detected

 

Additional information

Size

1 x 50 ml, 1 x 500 ml

18 reviews for Lipid-Depleted Fetal Bovine Serum (LD-FBS)

  1. azadi@live.ca –

    Review: we have purchased Lipid Depleted FBS , DMEM F12 …. they were good quality and decent price
    Have you purchased: Yes
    Time of submission: 29-Jan-2025 23:10:30
    Ease of placing the order: 5/5

  2. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 21-Feb-2025 15:30:00
    Ease of placing the order: ‘not given’

  3. Gregory-Mullen@ouhsc.edu –

    Review: We buy *all* of our cell culture media, fetal bovine sera, and lipid-depleted sera from PurMa. The quality of their reagents is absolutely top-notch, giving us reliable, repeatable results in our experiments. We highly recommend PurMa!
    Have you purchased: Yes
    Time of submission: 21-Feb-2025 15:46:22
    Ease of placing the order: 5/5

  4. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 24-Feb-2025 13:50:14
    Ease of placing the order: ‘not given’

  5. lrothblu@ouhsc.edu –

    Review: Serum worked beautifully. Cells grew well and had their usual cycle time. Tissue culture medium also worked very well.
    Have you purchased: It is my first Purchase
    Time of submission: 24-Feb-2025 13:53:22
    Ease of placing the order: 5/5

  6. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 27-Feb-2025 17:42:44
    Ease of placing the order: ‘not given’

  7. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 05-Mar-2025 16:31:44
    Ease of placing the order: ‘not given’

  8. michael-rudolph@ouhsc.edu –

    Review: I have worked directly with PurMa biologics on a number of projects ongoing in my lab. I find the media and the specialty Lipid Depleted Serum to be high quality and very consistent among batches, which is critical to our adipogenesis projects using primary stem cells. Also, the service from PurMa is outstanding for my lab.
    Have you purchased: Yes
    Time of submission: 05-Mar-2025 16:35:21
    Ease of placing the order: 5/5

  9. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 05-Mar-2025 17:29:13
    Ease of placing the order: ‘not given’

  10. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 10-Mar-2025 14:32:38
    Ease of placing the order: ‘not given’

  11. jkurien@hausbio.com –

    Review: We have been working with PurMa biologics for the past several years , we buy cell culture related media like DMEM F12 and EMEM and Fetal Bovine Serum . We appreciate the high quality and reasonable price, and great customer service.
    Have you purchased: Yes
    Time of submission: 12-Mar-2025 12:34:09
    Ease of placing the order: 5/5

  12. hyeungk1968@gmail.comthe –

    Review: I used Lipid Free horse serum of PurMa Biologics.
    The quality is great!
    Have you purchased: Yes
    Time of submission: 17-Mar-2025 12:09:58
    Ease of placing the order: 5/5

  13. jaya-krishnan@omrf.org –

    Review: I started my lab using the regular Gibco media components. We have shifted to Purma past 6 months and see no difference in the quality of our cell culture. Simply saying that Purma is cheaper, quicker to deliver and as good as Gibco. The best part was that Purma customized several media for our fish cell lines which was really helpful. Lastly, Seif walked through the customization of media over Zoom, that gave a lot of confidence for using those media solutions.
    Have you purchased: Yes
    Time of submission: 17-Mar-2025 14:22:58
    Ease of placing the order: 4/5

  14. tayyab-afzal@omrf.org –

    Review: We bought Premium FBS and DMEM cell culture media from PurMa Biologics. We are using them for several mammalian cell line and primary cell culture and the cell growth is perfectly fine. We are totally satisfied with the product quality. Importantly as local vendor, we can have the product available as per our demand ASAP.
    Have you purchased: Yes
    Time of submission: 18-Mar-2025 10:01:50
    Ease of placing the order: 5/5

  15. Fitzkr@gmail.com –

    Review: We purchase everything we need related to cell and tissue culture from PurMa, reagents like: DMEM, LL15, EMEM, FBS, trypsin, antibiotics mix100X and…
    Always great quality, fast processing and shipping.
    We are totally satisfied.
    Have you purchased: Yes
    Time of submission: 22-Mar-2025 10:47:00
    Ease of placing the order: 5/5

  16. vmadka@ouhsc.edu –

    Review:
    Have you purchased: Yes
    Time of submission: 24-Mar-2025 15:02:40
    Ease of placing the order: 4/5

  17. Anupam-Mandal@ouhsc.edu –

    Review: Excellent
    Have you purchased: Yes
    Time of submission: 24-Mar-2025 15:05:43
    Ease of placing the order: 5/5

  18. rohan-varshney@ouhsc.edu –

    Review: Reliable and dependable products. We purchased lipid-depleted FBS (LD-FBS) from PurMa Biologics and it worked great. We cross-checked the lipid content in the serum by GC-MS and Triglyceride assay and as advertised, did not find any lipids in the FBS.
    Have you purchased: Yes
    Time of submission: 24-Mar-2025 15:06:38
    Ease of placing the order: 5/5

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