MEM α-Modification with Nucleosides in Hanks’ Buffer

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MEM-α Modification with Nucleosides in Hanks’ Buffer (MEM-α-N-H)

MEM α-Modification with Nucleosides in Hanks’ Buffer

$49.10 – $1143.83

MEM α-Modification with Nucleosides in Hanks’ buffer provides a great nutritional culture environment for mammalian cells.  this media as well plays a key role in the process of selection for transfected DHFR-negative cells.

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Description

MEM α-Modification with Nucleosides in Hanks’ Buffer

Background

MEM α-Modification with Nucleosides  in Hanks’ buffer provides a great nutritional culture environment during mammalian cells.  This media, as well plays a key role in the process of selection of transfected DHFR-negative cells. Moreover, MEM-α-N-HBSS is widely used for a variety of suspension and adherent cells. Calcium, however, plays a crucial role in media as eliminating this ion facilitates the growth of cells in suspension cultures.

Ingredients

MEM-α with nucleosides is basically the modified version of MEM-α and contains:

  • Ribonucleosides
  • Deoxyribonucleosides
  • Non-essential amino acids.
  • Sodium pyruvate.
  • Lipoic acid.
  • Vitamin B12.
  • Biotin and,
  • Ascorbic acid.

PurMa™ MEM-α-N-HBSS contains HBSS buffer

The composition of HBSS buffer:

  1. Potassium Chloride
  2. Potassium Phosphate, monobasic
  3. Sodium Bicarbonate
  4. Sodium Chloride
  5. Sodium Phosphate, dibasic

Additionally, MEM-α-N-HBSS also Contains:

  • Low level of glucose (1.00 g/ liter)
  • Sodium bicarbonate (0.35 g/L)
  • 2 mM L-glutamine (292 mg/L)

PurMa Biologics manufactures 119 types of MEM-α-N-HBSS

Why PurMa Biologics? Why we stand behind our cell culture media

Our products are the outcome of 30 years of experience in cutting-edge science. We routinely manufacture over 1500 media. We have the most comprehensive collection of cell and tissue culture media. Moreover, We utilize %99 pure amino acids. This will significantly increase the consistency among your experiments. Considering the highest quality, our prices certainly are extremely reasonable.

Application

Furthermore,  MEM-α-N-HBSS is used for various cell types such as keratinocytes, primary rat astrocytes, as well as human melanoma cells. also, MEM-α-N-HBSS has been used for the isolation of mesenchymal stem cells, adipose-derived stem cells, and osteoblasts. Moreover, MEM α-Modification is widely used for primary osteoblast cultures.

Formulation

For complete formulation  click here as well as ” formulation Tab : MEM alpha with nucleoside in HBSS Buffer Formulation

References

  1. A previously functional tetracycline-regulated transactivator fails to target gene expression to the bone. Schmidt et al. BMC Res Notes. 2011 Aug 11;4:282. doi: 10.1186/1756-0500-4-282. PMID: 21835026.
  2. The growth of L-cells and Vero cells on an autoclavable MEM-peptone medium.  Keay L, Biotechnology and bioengineering, 19(3), 399-411 (1977-03-01
Parameter Specification
Appearance Red, clear liquid
pH 7.2 ± 0.1
Osmolality 275-360 mOsm/L
Endotoxin NMT< 2EU/mL
Mycoplasma Negative
Suitability  Suitable for mammalian cell culture
Additive Sodium pyruvate
Indicator Phenol red
Mycoplasma Detection Negative
Sterility Tested Sterile filtered using 0.22 µm filter
Form Liquid
Shipping Condition  Room temperature

 

Additional information

Condition

MEM-α-N (HBSS) Standard Formulation, MEM-α-N (HBSS) w/o Glutamine, MEM-α-N (HBSS) w/o Phenol Red, MEM-α-N (HBSS) w/o Sodium Bicarbonate, MEM-α-N (HBSS) w/o Glucose, MEM-α-N (HBSS) w/o Lipoic Acid, MEM-α-N (HBSS) w/o Glutamine, w/o Phenol Red, MEM-α-N (HBSS) w/o Glutamine, w/o Sodium Bicarbonate, MEM-α-N (HBSS) w/o Phenol Red, w/o Sodium Bicarbonate, MEM-α-N (HBSS) w/o Glutamine, w/o Phenol Red, w/o Sodium Bicarbonate, MEM-α-N (HBSS) 15 mM HEPES (3.6 g/L), MEM-α-N (HBSS) 25 mM HEPES (5.9 g/L), MEM-α-N (HBSS) w/o Sodium Pyruvate, MEM-α-N (HBSS) High Sodium Bicarbonate (0.85 g/L), MEM-α-N (HBSS) High Glucose (4.5 g/L), MEM-α-N (HBSS) With 15 mM HEPES (3.6 g/L), w/o Glucose, MEM-α-N (HBSS) With 25 mM HEPES (5.9 g/L), w/o Glucose, MEM-α-N (HBSS) 15 mM HEPES (3.6 g/L), High Glucose (4.5 g/L), MEM-α-N (HBSS) 25 mM HEPES (5.9 g/L), High Glucose (4.5 g/L), MEM-α-N (HBSS) 15 mM HEPES (3.6 g/L), High Glucose (4.5 g/L), High Sodium Bicarbonate (0.85 g/L), MEM-α-N (HBSS) 25 mM HEPES (5.9 g/L), High Glucose (4.5 g/L), High Sodium Bicarbonate (0.85 g/L)
Format

Liquid, Powder
Size

1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml, 1 x 10 L, 1 x 50 L, 10 X 1EZ Individually Packed, 50 X 1EZ Individually Packed

Quality Control

[vc-quality-control-tab]

Formulation

MEM-a-N in Hanks’ Buffer (HBSS) Formulation

SDS

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