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MEM Alpha Modification in Earle’s Buffer (MEM-α)

MEM Alpha Modification in Earle’s Buffer (MEM-Alpha)

(18 customer reviews)

MEM Alpha Modification in Earle’s Buffer (MEM-α) is a suitable nutrient for mammalian cell culture as well as selection for transfected DHFR-negative cells. Scientist around the globe use MEM -α Modification for primary osteoblast cultures as well as isolation of polymorphonuclear leukocytes (PMN)

SKU: N/A Categories: Cell Culture Media, MEM Alpha Modification in Earle’s Buffer (MEM-α) Tags: Cell Culture, Earle's Buffer, EBSS, glucose, MEM, MEM Alpha, MEM-α, Phenol Red, Sodium Bicarbonate, Standard, Without Phenol Red

Description

Background

MEM Alpha Modification in Earle’s Buffer (MEM-α) is certainly a suitable nutrient for mammalian cell culture as well as selection for transfected DHFR-negative cells. MEM -α Modification is widely used for primary osteoblast cultures as well as isolation of polymorphonuclear leukocytes (PMN) from umbilical cord blood. Eliminating calcium in this media facilitates the growth of cells in suspension cultures.

MEM -α  contains

  • Non-essential amino acids.
  • Sodium pyruvate.
  • Lipoic acid.
  • Vitamin B12.
  • Biotin and,
  • Ascorbic acid.
  • Also, MEM-α does not contain deoxyribonucleosides and ribonucleosides.
  • Moreover, PurMaTM MEM -α media in Earle’s buffer includes Earles’ buffer

The composition of Earle’s buffer, it contains:

  1. Calcium chloride
  2. Magnesium sulfate
  3. Potassium chloride
  4. Sodium bicarbonate
  5. Sodium chloride
  6. Sodium Phosphate, monobasic

Additionally, MEM Alpha Modification in Earle’s Buffer (MEM-α)

it contains:

  • Low level of glucose (1.00 g/ liter)
  • More importantly, Sodium bicarbonate (2.2 g/L) (if higher than 5% CO2 is used, a higher % of sodium bicarbonate is needed.
  • 2 mM L-glutamine (292 mg/L)’
  • Also, PurMa Biologics manufactures 118 types of MEM-α

Application

This media can also be used with a variety of suspension and adherent cells including keratinocytes, primary rat astrocytes as well as human melanoma cells. Furthermore, Alpha modification of Minimum Essential Medium Eagle has also been used for the isolation of mesenchymal stem cells, adipose-derived stem cells, and osteoblast.

Why PurMa Biologics? Why we stand behind our cell culture media.

Our products are the outcome of 30 years of experience in cutting edge science. We routinely manufacture over 1500 media. We have the most comprehensive collection of cell and tissue culture media. Moreover, we utilize %99 pure amino acids. This will significantly increase the consistency among your experiments. Considering the highest quality, our prices certainly are extremely reasonable.

Formulation

Complete formulation is in the ” Formulation tab” as well as here: MEM-alpha Formulation in Earle’s Buffer

References

  1. The growth of L-cells and Vero cells on an autoclavable MEM-peptone medium. Keay et al.  Biotechnology and bioengineering, 19(3), 399-411 (1977-03-01).
  2. Sphingosine-1-phosphate lyase (SGPL1) deficiency is associated with mitochondrial dysfunction. Maharaj et al. The Journal of steroid biochemistry and molecular biology, 202, 105730-105730 (2020-07-20)
Parameter Specification
Appearance Red, clear liquid
pH 7.2 ± 0.1
Osmolality 275-360 mOsm/L
Endotoxin NMT< 2EU/mL
Mycoplasma Negative
Suitability  Suitable for mammalian cell culture
Additive Sodium pyruvate
Indicator Phenol red
Mycoplasma Detection Negative
Sterility Tested Sterile filtered using 0.22 µm filter
Form Liquid
Shipping Condition  Room temperature

 

Additional information

Condition

MEM-α Standard Formulation, MEM-α w/o Glutamine, MEM-α w/o Phenol Red, MEM-α w/o Sodium Bicarbonate, MEM-α w/o Glucose, MEM-α w/o Lipoic Acid, MEM-α w/o Glutamine, w/o Phenol Red, MEM-α w/o Glutamine, w/o Sodium Bicarbonate, MEM-α w/o Glutamine, w/o Phenol Red, w/o Sodium Bicarbonate, MEM-α 15 mM HEPES (3.6 g/L), MEM-α 25 mM HEPES (5.9 g/L), MEM-α w/o Sodium Pyruvate, MEM-α High Glucose (4.5 g/L), MEM-α High Sodium Bicarbonate (3.7 g/L), MEM-α Low Sodium Bicarbonate (0.85 g/L)
Format

Liquid, Powder
Size

10 X 1000ml, 10 X 500ml, 1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml, 1 x 10 L, 1 x 50 L

18 reviews for MEM Alpha Modification in Earle’s Buffer (MEM-Alpha)

  1. azadi@live.ca –

    Review: we have purchased Lipid Depleted FBS , DMEM F12 …. they were good quality and decent price
    Have you purchased: Yes
    Time of submission: 29-Jan-2025 23:10:30
    Ease of placing the order: 5/5

  2. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 21-Feb-2025 15:30:00
    Ease of placing the order: ‘not given’

  3. Gregory-Mullen@ouhsc.edu –

    Review: We buy *all* of our cell culture media, fetal bovine sera, and lipid-depleted sera from PurMa. The quality of their reagents is absolutely top-notch, giving us reliable, repeatable results in our experiments. We highly recommend PurMa!
    Have you purchased: Yes
    Time of submission: 21-Feb-2025 15:46:22
    Ease of placing the order: 5/5

  4. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 24-Feb-2025 13:50:14
    Ease of placing the order: ‘not given’

  5. lrothblu@ouhsc.edu –

    Review: Serum worked beautifully. Cells grew well and had their usual cycle time. Tissue culture medium also worked very well.
    Have you purchased: It is my first Purchase
    Time of submission: 24-Feb-2025 13:53:22
    Ease of placing the order: 5/5

  6. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 27-Feb-2025 17:42:44
    Ease of placing the order: ‘not given’

  7. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 05-Mar-2025 16:31:44
    Ease of placing the order: ‘not given’

  8. michael-rudolph@ouhsc.edu –

    Review: I have worked directly with PurMa biologics on a number of projects ongoing in my lab. I find the media and the specialty Lipid Depleted Serum to be high quality and very consistent among batches, which is critical to our adipogenesis projects using primary stem cells. Also, the service from PurMa is outstanding for my lab.
    Have you purchased: Yes
    Time of submission: 05-Mar-2025 16:35:21
    Ease of placing the order: 5/5

  9. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 05-Mar-2025 17:29:13
    Ease of placing the order: ‘not given’

  10. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 10-Mar-2025 14:32:38
    Ease of placing the order: ‘not given’

  11. jkurien@hausbio.com –

    Review: We have been working with PurMa biologics for the past several years , we buy cell culture related media like DMEM F12 and EMEM and Fetal Bovine Serum . We appreciate the high quality and reasonable price, and great customer service.
    Have you purchased: Yes
    Time of submission: 12-Mar-2025 12:34:09
    Ease of placing the order: 5/5

  12. hyeungk1968@gmail.comthe –

    Review: I used Lipid Free horse serum of PurMa Biologics.
    The quality is great!
    Have you purchased: Yes
    Time of submission: 17-Mar-2025 12:09:58
    Ease of placing the order: 5/5

  13. jaya-krishnan@omrf.org –

    Review: I started my lab using the regular Gibco media components. We have shifted to Purma past 6 months and see no difference in the quality of our cell culture. Simply saying that Purma is cheaper, quicker to deliver and as good as Gibco. The best part was that Purma customized several media for our fish cell lines which was really helpful. Lastly, Seif walked through the customization of media over Zoom, that gave a lot of confidence for using those media solutions.
    Have you purchased: Yes
    Time of submission: 17-Mar-2025 14:22:58
    Ease of placing the order: 4/5

  14. tayyab-afzal@omrf.org –

    Review: We bought Premium FBS and DMEM cell culture media from PurMa Biologics. We are using them for several mammalian cell line and primary cell culture and the cell growth is perfectly fine. We are totally satisfied with the product quality. Importantly as local vendor, we can have the product available as per our demand ASAP.
    Have you purchased: Yes
    Time of submission: 18-Mar-2025 10:01:50
    Ease of placing the order: 5/5

  15. Fitzkr@gmail.com –

    Review: We purchase everything we need related to cell and tissue culture from PurMa, reagents like: DMEM, LL15, EMEM, FBS, trypsin, antibiotics mix100X and…
    Always great quality, fast processing and shipping.
    We are totally satisfied.
    Have you purchased: Yes
    Time of submission: 22-Mar-2025 10:47:00
    Ease of placing the order: 5/5

  16. vmadka@ouhsc.edu –

    Review:
    Have you purchased: Yes
    Time of submission: 24-Mar-2025 15:02:40
    Ease of placing the order: 4/5

  17. Anupam-Mandal@ouhsc.edu –

    Review: Excellent
    Have you purchased: Yes
    Time of submission: 24-Mar-2025 15:05:43
    Ease of placing the order: 5/5

  18. rohan-varshney@ouhsc.edu –

    Review: Reliable and dependable products. We purchased lipid-depleted FBS (LD-FBS) from PurMa Biologics and it worked great. We cross-checked the lipid content in the serum by GC-MS and Triglyceride assay and as advertised, did not find any lipids in the FBS.
    Have you purchased: Yes
    Time of submission: 24-Mar-2025 15:06:38
    Ease of placing the order: 5/5

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