Description

Roswell Park Memorial Institute 1640-MAX (RPMI-1640-MAX)

Roswell Park Memorial Institute 1640-MAX (RPMI-1640-MAX) is a commonly used media for Mammalian cell culture.

Advantages

The advantages of RPMI-1640-MAX over RPMI-1640 are:

• Higher PH Capacity
• Moreover, it contains 4 mM L-Alanyl-L-Glutamine (0.869 g/L) instead of L-Glutamine and therefore:
• Possess longer shelf life.
• More importantly, minimizes toxic ammonia build-up.
• Significantly improves cell viability and growth.
• Remains stable across a wide range of temperatures.
• Improves the culture of primary cells.

Mechanism

Although L-glutamine is a vital amino acid, it degrades, resulting in the generation of toxic ammonia and pyrrolidine carboxylic acid. One way to minimize L-glutamine degradation in media is to gradually add these amino acids to your cell culture. However, keeping track of time to keep the amount of L-glutamine at the same level is tedious, if not impossible.

Alternatively, L-alanyl-L-glutamine can be used because it is much more stable in aqueous solutions. More importantly, it does not easily degrade and gradually releases aminopeptidases. As a result, L-glutamine and L-alanine are then used by the cells for protein production in the TCA cycle.

In addition to our Max formulation products, we manufacture PurMa^TM GluaSup (Cat# P3S210 559) is a combination of L-alanyl-L-glutamine which prevents degradation of L-glutamine.

Contents

Roswell Park Memorial Institute 1640-MAX (RPMI-1640-MAX) contains:

• 5 mM sodium pyruvate
• High glucose (4.5g/L)
• Non-Essential Amino Acids
• 3.7 g/L Sodium Bicarbonate; PurMa Biologics Manufactures several types of RPMI-1640-MAX  based on the amount of Sodium Bicarbonate and HEPES (PH capacity)
• Phenol Red

RPMI-1640-MAX contains no proteins, lipids, or growth factors. Therefore, it requires supplementation, commonly with 10% Fetal bovine Serum (FBS).

Formulation

For complete formulation click here: RPMI-1640 MAX Formulation

References

1. Culture of normal human leukocytes. Moore GE, et al. 1967 Feb 20;199(8):519-24. PMID: 4960081
2. Leukocytes as carriers in the transmission of bovine leukemia: certain morphologic and functional characteristics of cultured leukocytes from normal and leukemic cattle. Dunne HW, et al. Am J Vet Res. 1970 Apr;31(4):597-617. PMID: 5437104.
3. Long-term culture of human leukaemic leukocytes. Pössnerová V, et al. 1970;17(5):513-23. PMID: 5274436