Description

Dulbecco’s Modified Eagle Medium: Nutrient Mixture F-12 (DMEM/F-12) provides superior quality, consistency, and great control in mammalian cell culture. DMEM-F12 contains a 1:1 mixture of DMEM and F-12. This formulation combines DMEM’s high concentrations of glucose, amino acids, and vitamins as well as much more variation of salts with F-12’s wide variety of components. In some types of PurMaTM DMEM: F-12, the concentration of sodium bicarbonate  used is lower than DMEM, therefore requires a 5–10% CO2 environment to maintain physiological pH. DMEM: F-12 is better than DMEM and advanced IMDM on MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts. DMEM-F12 contains no proteins, lipids, or growth factors. Therefore, it requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). In general, DMEM: F-12 is more enriched but contains the following major changes compared to DMEM:

  • 2.5 mM L-glutamine
  • 0.5 mM sodium pyruvate
  • 30mM NaHCO3 (2.438g/L)

 Regular DMEM / F-12 Contains no proteins, lipids, or growth factors. Therefore, it requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). it contains:

  • High glucose
  • Non-Essential Amino Acids
  • Sodium pyruvate
  • Phenol Red
  • L-glutamine

Formulation: Complete formulation is here: DMEM-F12 Formulation

References

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  2. Plaque production by the polyoma virus. DULBECCO R, FREEMAN G. 1959 Jul;8(3):396-7. doi: 10.1016/0042-6822(59)90043-1. PMID: 13669362.
  3. The nucleic acid of polyoma virus. Smith, J.D., Freeman, G., Vogt, M., et al., (1960) The nucleic acid of polyoma virus. Virology 12:185
  4. Putrescine: Essential factor for in vitro proliferation of Babesia bovis. Rojas-Martínez C, Rodríguez-Vivas RI, Figueroa Millán JV, Acosta Viana KY, Gutiérrez Ruiz EJ, Álvarez Martínez JA. Exp Parasitol. 2017 Apr; 175:79-84. doi: 10.1016/j.exppara.2017.01.010. Epub 2017 Jan 30. PMID: 28153804.
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  2. Anterior pituitary influence on adipokine expression and secretion by porcine adipocytes.
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  4. Optimization of culture media to enhance the growth of tissue engineered cartilage. Bajic A, Tarantino R, Chiu LLY, Duever T, Waldman SD.Biotechnol Prog. 2020 Sep;36(5): e3017. doi: 10.1002/btpr.3017. Epub 2020 May 26.PMID: 32394623.
  5. A cell culture medium that supports the differentiation of human retinal pigment epithelium into functionally polarized monolayers. Hu J, Bok D. Mol Vis. 2001 Feb 7;7:14-9. PMID: 11182021
  6. Amino acid abundance and composition in cell culture medium affects trace metal tolerance and cholesterol synthesis. Rawat J, Bhambri A, Pandey U, Banerjee S, Pillai B, Gadgil M.Biotechnol Prog. 2023 Jan;39(1):e3298. doi: 10.1002/btpr.3298. Epub 2022 Sep 30.PMID:
  7. Evaluation of a simple protein free medium that supports high levels of monoclonal antibody production. Qi YM, Greenfield PF, Reid S. 1996 Jun;21(2):95-109. doi: 10.1007/BF02215660. PMID: 22358660.
Parameter Specification
Appearance Red, clear liquid
pH 7.2 ± 0.1
Osmolality 275-360 mOsm/L
Endotoxin NMT< 2EU/mL
Mycoplasma Negative
Suitability  Suitable for mammalian cell culture
Additive Sodium pyruvate
Indicator Phenol red
Mycoplasma Detection Negative
Sterility Tested Sterile filtered using 0.22 µm filter
Form Liquid
Shipping Condition  Room temperature